Twilight Sparkle's human counterpart appears and is mentioned throughout the My Little Pony Equestria Girls film series. She is a student at Canterlot High School, a former student of Crystal Prep Academy, and the owner of Spike the Dog. She is named Sci-Twi in some merchandise and other depictions. Her alter ego, Midnight Sparkle, first appears as an antagonist in My Little Pony Equestria Girls: Friendship Games.
- she's used to be midnight sparkle but now her clone of midnight sparkle is her enemy
- She's Tails girlfriend, Cream's Best Friend and bambi's friend
- Her Pony Counterpart's Adopted Father Is Lugia
- Her Best Friends are Star Butterfly (which they're childhood friends) and Cream